The KNIME Image Processing Extension adds new image types and functions to process and analyze images to KNIME. The corresponding nodes allow one to read more than 100 different kinds of images (thanks to the BioFormats API1), to apply well known methods for the preprocessing and to perform image segmentation. Most of the included nodes operate on multidimensional image data (e.g. videos, 3D images, multi-channel images or even a combination of them), which is made possible by the internally used ImgLib2-API2. Further details and news regarding the KNIME Image Processing Extension can be found at http://tech.knime.org/community/image-processing. This manual is targeted to users that are already familiar with KNIME and want to learn the usage of the KNIME Image Processing Extension. If you are not familiar with the basic principles of KNIME we highly recommend to get started with the KNIME basics first, e.g. by reading http://tech. knime.org/getting-started