3 min at 94 C, followed by 12 cycles of 45 s at 94 C,
45 s at 65 C and 1 min at 72 C; the annealing temperature
was decreased by 0.7 C per cycle from a starting point of 65 C during this stage (Touchdown), with a final
round of 25 cycles of 94 C for 45 s, 56 C for 45 s, 72 C
for 1 min and a final extension of 72 C for 1 min.