The phaC1 gene with ribosome binding site was amplified from genomic DNA of P. putida KT2440 by PCR employing Pfu DNA polymerase and a pair of primers amC1-F/amC1-R. The PCR products of phaC1 were purified, digested with HindIII/XhoI, purified and inserted into purified HindIII/XhoI-hydrolyzed pBluescript II KS (+), resulting in plasmid pBlueC1.