Abstract A protocol for jasmine rice authentication was
established in order to determine whether a Thai jasmine
rice product meets legal requirement. Both RAPD and
SCAR methods were used on the basis of previous study of
jasmine-rice speciWc genome characteristics. Two RAPD
markers, RI2-449 and RI5-1107, were selected and SCAR
primer pairs were designed for RI5-1107. Twenty-four rice
cultivars, which represented typical jasmine rice and nonjasmine
rice were analyzed to conWrm the speciWcity and
reproducibility of DNA method. The purity of jasmine rice
was calculated by analyzing every single kernel from test
sample. Five in-house prepared samples were quantiWed to
prove the accuracy of DNA method, and 15 imported samples
were analyzed by DNA method as well as physical/
chemical method to conWrm the consistency between DNA
method and traditional method. Based on the standard deviation
obtained by comparing DNA method and traditional
method, a testing threshold for jasmine rice was decided.