blender. Samples for SSP analysis were prepared by homogenising
fish mince (2 g) with 38 ml of 5% NaCl solution (buffered with
0.02 M NaHCO
3
, pH 7.2) for 2 min using a laboratory blender.
WSP and SSP were extracted by stirring in an ice bath for 2 h and
then centrifuging at 8228
g
for 10 min at 4
°
C using a centrifuge
(Eppendorf Co., 5804R, Hamburg, Germany). The protein content
of the supernatant was assayed by the Bradford method (
Löffler
& Kunze, 1989
).