Otherwise,
T. spiralis larvae and adults localize at the crypt-villus junction after
infecting the host [5]. This conclusion was confirmed in our study
by HE staining (Figure 5A) and IF test (Figure 5B, 5C and 5D) of
the small intestines of mice infected with T. spiralis. Therefore,
mouse intestinal crypts were isolated and cultured in vitro for
harvesting IECs in this study. The monolayers of cultured IECs
invaded by T. spiralis will provide the natural model of invasion to
mimic the normal intestinal epithelium in vivo.