The plant material
was botanically characterized by Professor Smaoui (Borj Cedria
Biotechnology Center, Tunisia) according to the morphological
description presented in Tunisian flora (Pottier–Alapetite 1979).
Voucher specimens (P.l.01 and P.l.02) were deposited in the Laboratory
of Biotechnology Applied to Agriculture (National Agricultural
Research Institute of Tunisie, INRAT). The harvest material was airdried
at 20–25 ◦C (room temperature) for two week