The first treatment consisted of a mix of microorganisms isolated from bioflakes at a concentration of 1 × 104 CFU·mL−1 (strains used: Bacillus thuringiensis CPQBA 571-12 DRM 06, Bacillus licheniformis CPQBA 571- 12 DRM 07, B. licheniformis CPQBA 571-12 DRM 08 and Bacillus cereus CPQBA 571-12 DRM 09) and the control group, without adding microorganisms,
in quadruplicate.