Clayloamsoilsampleswerefortified with fipronil @0.50,0.75,
1.00,1.25and1.50mgkg−1 along with45107 B. thuringiensis
microbecells.Fromeachfortified (insecticide+B. thuringiensis) sample,
50gsoilsamplewastakenand filledinplasticcup.Thesamplesof
clayloamsoilwereanalyzedat7,14,28,35,42,49and56daysafter
initiationofthisexperiment.Themaximumresiduesof fipronil andits
metaboliteswerefoundtobe0.38,0.60,0.72,0.81and1.13mgkg−1 in
soilcollectedat7daysaftertheapplicationof fipronil@0.50,0.75,
1.00,1.25and1.50mgkg−1, respectively.Theseresidueswere
degradedto0.08,0.14,0.22,0.31and0.40mgkg−1, respectivelyin
21days.Amongmetabolites,sulfidewasfoundtobemainmetabolite
followedbysulfoneandamide.Desulfinylmetabolitewasnot
producedinanyofthesample.Nometabolitesweredetectedwhen
the soilwasfortified with fipronil@0.50mgkg−1. Noresidueswere
detectedat28daysaftertheapplicationofpesticidewhenthesoil
samplewasfortified with@0.50mgkg−1. Whenthesoilsamples
werefortified with fipronil@0.75,1.00and1.25mgkg−1, residues
werenotdetected35daysaftertheapplicationofpesticide.But,when
the soilsamplewasfortified with fipronil @1.50mgkg−1, residuesof
fipronilpersistedupto35days.Butnoresiduesweredetected42days
aftertheapplicationoftheinsecticides.Theseresultsshowthatthe
bacterium B. thuringiensis has agreatroleinthebioremediationof
fipronilcontaminatedsoils(Fig. 2).Thesestudiesclearlydemonstrated
the roleofthesemicrobesasthepersistenceof fipronilwasfoundto
be verylessascomparedtounamendedsoilswhere fipronilwas
found topersistformorethan56days(Fig. 2).
Thestudiesareinagreementwiththoseof Zhuetal.,2004 who
hasalsoreportedfasterdegradationof fipronilbysoilmicrobes.
Thedegradationof fipronilinnon-sterileclayloamsoilwasmainly
influencedbythesoilmicrobes.Thehalf-livesinnon-sterileclay
Clayloamsoilsampleswerefortified with fipronil @0.50,0.75,1.00,1.25and1.50mgkg−1 along with45107 B. thuringiensismicrobecells.Fromeachfortified (insecticide+B. thuringiensis) sample,50gsoilsamplewastakenand filledinplasticcup.Thesamplesofclayloamsoilwereanalyzedat7,14,28,35,42,49and56daysafterinitiationofthisexperiment.Themaximumresiduesof fipronil anditsmetaboliteswerefoundtobe0.38,0.60,0.72,0.81and1.13mgkg−1 insoilcollectedat7daysaftertheapplicationof fipronil@0.50,0.75,1.00,1.25and1.50mgkg−1, respectively.Theseresiduesweredegradedto0.08,0.14,0.22,0.31and0.40mgkg−1, respectivelyin21days.Amongmetabolites,sulfidewasfoundtobemainmetabolitefollowedbysulfoneandamide.Desulfinylmetabolitewasnotproducedinanyofthesample.Nometabolitesweredetectedwhenthe soilwasfortified with fipronil@0.50mgkg−1. Noresiduesweredetectedat28daysaftertheapplicationofpesticidewhenthesoilsamplewasfortified with@0.50mgkg−1. Whenthesoilsampleswerefortified with fipronil@0.75,1.00and1.25mgkg−1, residueswerenotdetected35daysaftertheapplicationofpesticide.But,whenthe soilsamplewasfortified with fipronil @1.50mgkg−1, residuesoffipronilpersistedupto35days.Butnoresiduesweredetected42daysaftertheapplicationoftheinsecticides.Theseresultsshowthatthebacterium B. thuringiensis has agreatroleinthebioremediationoffipronilcontaminatedsoils(Fig. 2).Thesestudiesclearlydemonstratedthe roleofthesemicrobesasthepersistenceof fipronilwasfoundtoมี verylessascomparedtounamendedsoilswhere fipronilwasพบ topersistformorethan56days(Fig. 2)Thestudiesareinagreementwiththoseof Zhuetal. 2004 ที่hasalsoreportedfasterdegradationof fipronilbysoilmicrobesThedegradationof fipronilinnon-sterileclayloamsoilwasmainlyinfluencedbythesoilmicrobes Thehalf-livesinnon-sterileclay
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clayloamsoilsampleswerefortified กับฟิโปรนิล 0.50,0.75
@ , 1.00,1.25and1.50mgkg − 1 ตาม with45 107 B . thuringiensis
microbecells.fromeachfortified ( ยาฆ่าแมลง B . thuringiensis ) ตัวอย่าง
50gsoilsamplewastakenand filledinplasticcup . thesamplesof
clayloamsoilwereanalyzedat7,14,28,35,42,49and56daysafter initiationofthisexperiment.themaximumresiduesof ฟิโปรนิลและ metaboliteswerefoundtobe0.38, 0.60 ,0.72,0.81and1.13mgkg − 1 ใน
@
1.00,1.25and1.50mgkg 0.50,0.75 soilcollectedat7daysaftertheapplicationof ฟิโปรนิล , − 1 , − 1 ) theseresidueswere
degradedto0.08,0.14,0.22,0.31and0.40mgkg , 21days amongmetabolites respectivelyin
. ,
sulfidewasfoundtobemainmetabolite followedbysulfoneandamide . desulfinylmetabolitewasnot nometabolitesweredetectedwhen
producedinanyofthesample .การ soilwasfortified กับฟิโปรนิล @ 0.50mgkg − 1 noresidueswere
samplewasfortified detectedat28daysaftertheapplicationofpesticidewhenthesoil @ 0.50mgkg − 1 whenthesoilsamples
werefortified กับฟิโปรนิล @ 0.75,1.00and1.25mgkg − 1 , ตกค้าง
werenotdetected35daysaftertheapplicationofpesticide แต่เมื่อ soilsamplewasfortified กับฟิโปรนิล @ 1.50mgkg − 1 , residuesof
fipronilpersistedupto35days .butnoresiduesweredetected42days
aftertheapplicationoftheinsecticides . theseresultsshowthatthe แบคทีเรีย B . thuringiensis ได้ agreatroleinthebioremediationof
fipronilcontaminatedsoils ( รูปที่ 2 ) thesestudiesclearlydemonstrated
เป็น roleofthesemicrobesasthepersistenceof fipronilwasfoundto fipronilwas verylessascomparedtounamendedsoilswhere พบ topersistformorethan56days
( รูปที่ 2 )thestudiesareinagreementwiththoseof zhuetal . 2004 ที่ hasalsoreportedfasterdegradationof fipronilbysoilmicrobes
.
thedegradationof fipronilinnon sterileclayloamsoilwasmainly
influencedbythesoilmicrobes.thehalf-livesinnon-sterileclay
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