A. succinogenes ATCC 55618 was obtained from the American Type Culture
Collection. Cells were grown in 100mL sealed anaerobic bottles containing 50mL
medium. The medium for inoculum cultures was composed of (per liter): 20 g glucose,
10.0 g yeast extract, 5.0 g corn steep liquid (dry power), 2.0 g NaH2PO4·H2O,
3.0 g K2HPO4 and 20.0 gMgCO3. The medium was heat sterilized at 121 ◦C for 15 min.
The anaerobic bottles were inoculated with 1mL of a −80 ◦C stored glycerol stock
culture and incubated at 38 ◦C for 16 h.