BS11 identification and its characteristics
BS11 is a Gram-positive, rod-shaped bacteria with the cell size of ~0.45-0.55 x 2.5-3.5 m (width x length) and canform central spore as shown in Figure 1A and B, respectively.
Biochemical characteristics of BS11 were identified using the test kits of api 20E and api
50 CHB. Medium and physiological properties examined using microbiological methods
were indicated in Table 1. After analysis with the APILAB Plus software, percentages of similarity of BS11 to Bacillus subtilis is 99.9%. Genetic analysis using 16S rRNA gene was performed to identify BS11. The sequence of 16S rDNA from
BS11 shows close relatedness with Bacillus subtilis, and its sequence (1513 base pair) was submitted to NCBI gene bank and obtained the accession number of the Genbank, GU166746.
Phylogenetic tree showed close similarity of BS11 with Bacillus subtilis (Figure 2). Results of the biochemical analysis were in agreement of 16S r-RNA analysis for BS11. Therefore, BS11 is mostly classified as Bacillus subtilis. Among six
.
In order to identify the specific markers of BS11, all
Bacillus
spp. were analyzed by RAPD-PCR using indepen-
dently prepared template DNAs in triplicate
BS11 identification and its characteristicsBS11 is a Gram-positive, rod-shaped bacteria with the cell size of ~0.45-0.55 x 2.5-3.5 m (width x length) and canform central spore as shown in Figure 1A and B, respectively.Biochemical characteristics of BS11 were identified using the test kits of api 20E and api50 CHB. Medium and physiological properties examined using microbiological methodswere indicated in Table 1. After analysis with the APILAB Plus software, percentages of similarity of BS11 to Bacillus subtilis is 99.9%. Genetic analysis using 16S rRNA gene was performed to identify BS11. The sequence of 16S rDNA fromBS11 shows close relatedness with Bacillus subtilis, and its sequence (1513 base pair) was submitted to NCBI gene bank and obtained the accession number of the Genbank, GU166746.Phylogenetic tree showed close similarity of BS11 with Bacillus subtilis (Figure 2). Results of the biochemical analysis were in agreement of 16S r-RNA analysis for BS11. Therefore, BS11 is mostly classified as Bacillus subtilis. Among six.In order to identify the specific markers of BS11, allBacillus spp. were analyzed by RAPD-PCR using indepen-dently prepared template DNAs in triplicate
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