For each of 4 trials, 50 nest-clean broiler hatching eggs were obtained from a commercial hatchery 2 d after lay.
The eggs were from breeder hens at 58, 51, 28, and 51 wk of age (trials 1 to 4, respectively).
The eggs were removed from the hatchery egg holding room on the morning of each trial and transported to the laboratory on cardboard flats, with each flat in an individual plastic bag.
On arrival, the eggs were allowed to warm to laboratory room temperature for 2 h.
Eggs had not been sprayed or otherwise sanitized before arrival or removal from the hatchery.
For each of 4 trials, 50 nest-clean broiler hatching eggs were obtained from a commercial hatchery 2 d after lay. The eggs were from breeder hens at 58, 51, 28, and 51 wk of age (trials 1 to 4, respectively). The eggs were removed from the hatchery egg holding room on the morning of each trial and transported to the laboratory on cardboard flats, with each flat in an individual plastic bag. On arrival, the eggs were allowed to warm to laboratory room temperature for 2 h. Eggs had not been sprayed or otherwise sanitized before arrival or removal from the hatchery.
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