Table 1 summarizes the kinetics of cell growth and acetic acid production when cells
cultivation in shake flask and bioreactor under different cultivation conditions. As shown,
optimization of growth medium resulted in significant increase in volumetric acetic acid
production from only 22 g/L upto 55 g/L concomitant with 3 fold increases in acid
production rate [Qp]. Further improvement in the production process was achieved by
process scaling up from shake flask to 16-L stirred tank bioreactor. Maximal acid production
of about 76 g/L was achieved in non pH controlled culture after only 44 h cultivation. This
process shows high potential for industrial scale production of acetic acid using glucose
based and alcohol free medium.