20 μl, were mixed with 1 ml of diluted ABTSradical dot+ solution and incubated at 30 °C. The absorbance at 734 nm was read every minute for 30 min using the Safire microplate reader. Ethanol (95%) was used as a blank. Trolox with concentrations from 0 to 500 μM was used as a standard. The free-radical-scavenging activity was expressed as μmoles of Trolox per gram of sample (μmol TE/g fw or dw).