The Ubi1:AtNPR1 chimeric gene construct (plasmid
pJS406) contains a cDNA, which encompasses the translated
plus the 5′ and 3′ transcribed but nontranslated regions of the
AtNPR1 gene from the Arabidopsis ecotype Dijon-17 (Zhou et
al. 2000). In planta expression of AtNPR1 is driven from the
ubiquitously expressed maize Ubiquitin1 (Ubi1) promoter,
with the maize Ubi1 intron added to enhance stability of transcript
The 3′ nontranscribed region of the Agrobacterium tumefaciens nopaline synthase (nos)
gene provides the transcription termination information. The
gene construct Ubi1:bar in the plasmid pAHC20 expresses the bar gene from the Ubi1 promoter-intron
. The bar gene confer resistance to the herbicide
glufosinate Embryogenic calli were used for cotrans