From the present study it was concluded that several factors (cultivar, kind and concentration of growth
regulators) can influence the micropropagation, vitrification and rooting of carnation. There was a significant
difference in the average number of regenerated shoots between the cultivars. Eskimo Mogr produced more
regenerated shoots than Innove Orange Bogr. The use of Kin resulted in a significantly lower shoot
multiplication compared to BAP. Increasing the concentration of cytokinin increased the number of
regenerated shoots and also vitrification rate. Application of BAP in the medium compared with Kin
stimulates the rate of this phenomenon. The greatest shoot formation was obtained on media with 4 mg/l
BAP, however in this medium vitrification was very high. Since BAP has better effect on shoot regeneration,
it is suggested that lower concentration of this growth regulator can be used for obtaining the desirable shoot
regeneration with less vitrification rate. Rooting of regenerated shoots was depended on genotype and the
best rooting response took place on 0.5 to 1.5 mg/l of each auxins. The rooted plantlets were successfully
acclimatized and transferred to greenhouse (with a 90% success), where they displayed normal growth.