Among different biosurfactants and producing microorganisms,the production of lipopeptide biosurfactants, mostly produced byBacillus sp., is highlighted due to its considerable efficiency andcommercial interest [1]. The most known lipopeptide biosurfac-tant are divided in three groups (surfactins, iturins and fengycins),and each group consists on isoforms and homologous series. Sur-factin is one of the most powerful biosurfactants and it has gainedattention for industrial application, since it can lower the surfacetension of water from 72 to 27 mN m−1at low concentrations [8],and also due to its antimicrobial, antiviral and antitumor activities