2.6. Quantification of Xcc
Viable bacteria in the inoculated areas were estimated at 14
dpi for detached leaf assay and 20 for attached leaf assay. Leaf
disks (6 mm diameter) were excised from three infiltrated sites
and ground in 1 mL of PBS buffer using a glass homogenizer.
Serial dilutions of bacterial suspension were plated on KCC medium
(nutrient agar plus kasugamycin 16 mg L1, cephalexin
16 mg L1, and chlorothalonil 12 mg L1) as previously described
(Francis et al., 2010).