Upon scale-up at 300 kg or 750 kg, the mixing of the API X per- formed differently from the 20-kg scale. Fig. 6 shows the maximum dif- ference in potency across the mixing steps at 300 kg and 750 kg with the 5% w/w drug load and the 20-kg scale batch at 5% w/w is plotted in the same graph for comparison. Blend 1 had a smaller MD at commercial scales (b20%), suggesting improved yet still ineffective macro-mixing upon scale up. This distinction between scales on blend 1 indicates that macro-mixing in the first blending step is more effective at larger scales due to increased shear provided upon scale up [10].