A novel molecularly imprinted stir bar (MIP-SB) was developed with melamine (MA) as the template
molecule in this study. The sorptive capacity of MIP-SB was nearly three times over that of
non-imprinted stir bar (NIP-SB). The MIP-SB presented much better selectivity than NIP-SB when used
to absorb MA and its analogues. An analytical method to determine MA in powdered milk was established
by combining MIP-SB sorptive extraction with HPLC. The liner range was 0.0631–12.6 ng/mL with
good correlation coefficient of 0.9983, and the limit of detection was 0.0127 ng/mL based on three times
ratio of signal to noise. This method was successfully applied to the determination of MA in powdered
milk with satisfactory results. The method built is simple and suitable for the determination of MA in
milk products which is of great significance for quality control of milk products.