Conical centrifuge tubes
containing 5 mL FC were inoculated in triplicate with 0.5 mL of each
spoilage culture and incubated anaerobically at ambient temperature
(25 C) along with triplicate non inoculated FC controls. A
duplicate set of treatments was incubated under static aerobic
conditions at 25 C. Samples were aseptically withdrawn at 9, 22,
51, 77, 96, and 146 days of incubation and stored at 80 C until
analysis.