Area 300: Enzymatic hydrolysis and fermentation. Enzymatic hydrolysis is initiated in a high-solids continuous reactor using a cellulase enzyme prepared on-site. The partially hydrolyzed slurry is next batched to one of several parallel bioreactors. Hydrolysis is completed in the batch reactor, and then the slurry is cooled and inoculated with the cofermenting microorganism Zymomonas mobilis. After a total of five days of sequential enzymatic hydrolysis and fermentation, most of the cellulose and xylose have been converted to ethanol. The resulting beer is sent to the product recovery train (Area 500).