One-step desolvation. Gelatin (200 mg) was
dissolved in distilled water (10 mL) under constant
heating at 40 ± 1°C, pH 3 (by adding 0.1 M HCL).
BSA was added, followed by dropwise addition of
acetone (30 mL) to form GNP. At the end of the
process, glutaraldehyde solution (25% v/v in distilled
water [100 μL]) was added as a cross-linking agent,
and the solution was stirred for 30 min. Dispersion was
centrifuged at 11790 RCF for 30 min. The particles
were purified by threefold centrifugation and
ridispersion water. After the last ridispersion,
fabricated particles were freeze-dried, and then white
freely flowing powder of BSA-loaded GNP was
obtained.