Coconut milk is an emulsion which is stabilized by naturally occurring proteins. The main objective of the
present work is to explore different methods employing thermal, pH, chilling, enzyme treatments and
combination of enzyme treatments followed by chilling and thawing for effective destabilization of the
coconut milk emulsion. Stability of emulsion is evaluated by measuring the creaming index and observed
for the changes in structure of oil droplets, using phase contrast microscope. Combination of treatments
(enzyme treatment at 37 C followed by chilling and thawing) of coconut milk emulsion has resulted in
highest yield of 94.5%. Physico-chemical properties and fatty acid compositions are evaluated for coconut
oil obtained by combination of treatments and compared with that of commercial coconut oil. It is found
that the oil obtained by combination of treatments is low with respect to free fatty acids and peroxide
value and high in lauric acid content.