Optimum concentration of selected factors obtained as response of statistical
experimental design were 0.8% (v/v) glycerol waste, 0.26% (w/v) BHM and 1.25%OD as an inoculum
for the maximum PHA production. The suggested model was validated and maximum 1.36 g/L of PHA
production was obtained after 96 h. PHA production of 1.87 g/L was achieved in 5 L (working volume 3 L)
lab scale bioreactor with the suggested media components by RSM (Response Surface Methodology).
Characterization of the PHA by NMR spectroscopy revealed that the polymer was a hetromonomer of
(R)-3-hydroxybutyrate and medium chain length 3HA[(R)-3-hydroxyalkanoate] monomers.