Immunological assessment: On 43 day of age, six
rd
birds from each dietary treatment were injected intra
dermally in the right wattle with 100 µg of
Phytohaemaglutinin-P (PHA-P) in 0.1 ml of normal
saline solution to measure the cellular immune
response by Cutaneous Basophilic Hyper Sensitivity
(CBH) test (Edelman et al., 1986). The thickness of
wattle was measured using micrometer before
inoculation and 24 h post inoculation and CBH
response was calculated using the formula: