Methods: A total of 47 clinical mold isolates were used in this study, including Aspergillus
and Trichophyton. All isolates were identified by phenotypic properties, such as growth
rate, colony morphology, and reproductive structures. PCR and direct sequencing, targeting
the internal transcribed spacer (ITS) region, the D1/D2 region of the 28S subunit, and
the ß-tubulin gene, were performed using primers described previously. Comparative sequence
analysis by using the GenBank database was performed with the basic local alignment
search tool (BLAST) algorithm.
Methods: A total of 47 clinical mold isolates were used in this study, including Aspergillusand Trichophyton. All isolates were identified by phenotypic properties, such as growthrate, colony morphology, and reproductive structures. PCR and direct sequencing, targetingthe internal transcribed spacer (ITS) region, the D1/D2 region of the 28S subunit, andthe ß-tubulin gene, were performed using primers described previously. Comparative sequenceanalysis by using the GenBank database was performed with the basic local alignmentsearch tool (BLAST) algorithm.
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