The same relationship between Tleaf and gas-exchange measurements were found using the values of Tleaf–air(Fig. 4D–F). Although these relations were not similar for each timing measurement, there was a grouping of dataset , mainly for the data from the beginning of the experiment (0 DAT) in relation to the rest. When these same relationships were calculated using the average val-ues, as described in the model of Eq. (1), the optimum ranges of Tleaf–air related to gas exchange measurements were identified(Fig 5, Table 4), that is, those in which a minimal increase was detected in gas-exchange responses. According to these results,Tleaf–air values below to −2◦C would be accompanied with maxi-mum values of An and gs the maximum values of E would be reached for values of Tleaf–air close to −4◦C.