2.4.5.3. DPPH assay.
The value of DPPH (millimolar Trolox equivalents,
mMTE) measures the antioxidant capacity of a given substance,
as compared to the standard (Trolox). The method used
was as described by Brand-Williams, Cuvelier, and Berset (1995).
The reaction was begun by adding 50 lL of a suitable dilution of
sample to 1.45 mL of DPPH coloured radical. The sample was incubated
for 30 min at room temperature (20 C). Absorbance, A, was
measured at the wavelength of 515 nm.