The mixture was sonicated in an ultrasound water bath (Bandelin Sonorex,
RK103H, 140/560W, 35 kHz, Germany) for 40 min to ensure maximum
back extraction of analytes and then centrifuged for 3 min
at 8000 rpm. Two distinct layers were formed, the upper was surfactant-
rich phase, and the lower phase was organic solvent-rich
(chloroform), the analytes were remained in the chloroform phase.