Phytotherapy of chlorophyllin formulations against Fasciola gigantica infected Lymnaea
acuminata under sunlight exposure was highly toxic against redia and cercaria larvae. Binary
combinations (1:1 ratio) of chlorophyllin (CHL) + freeze dried cow urine (FCU) were more
toxic against cercariae (8 h LC50: 9.6 mg L−1
) than single treatment with chlorophyllin (8 h
LC50: 12.6 mg L−1
) in sunlight. The larvicidal activity of sunlight exposed CHL against rediae
(8 h LC50: 13.5 mg L−1
) and cercariae (8 h LC50: 12.6 mg L−1
) was more pronounced than laboratory
conditions CHL treatment (rediae- 8 h LC50: 305.9 mg L−1
; cercariae- 8 h LC50:
765.4 mg L−1
). Larvicidal activity of FCU was less than CHL and CHL + FCU against both
redia and cercaria. Chlorophyllin and its formulations were more toxic against redia and cercaria
larvae in sunlight than laboratory conditions. CHL and its different formulations may be used
as potent larvicides against Fasciola gigantica larvae. Chlorophyllin formulations will be economical,
ecologically sounder and their use in aquatic environment will be safe.