The defatted samples were then air dried overnight to evaporate the n hexane residue and then extracted twice using 5 ml
of a mixture of 70% (v/v) acetone (Merck) 29.8% (v/v) distilled water (RCI Labscan) and 0.2% (v/v) acetic acid (Merck) for 30 min using a ultrasonic bath (FALC, Treviglio, Italy).