3. Results and discussion
3.1. Two dimensional thin layer chromatography
(preliminary test)
TLC plates checked under UV with and without the
presence of ammonia, showed yellow, orange, violet
and deep purple spots. According to Mabry et al.
(1970), deep purple or violet spots turning to yellow in
the presence of NH3 fumes indicate the presence of flavones
with 5-OH and 40-OH or 3-OH substituted flavonols
with 5-OH and 40-OH and some 5-OH flavanones
and 40-OH chalcones lacking B-ring hydroxyl groups.
Deep purple color spots not changing in the presence
of NH3 fumes show the presence of 5-OH flavones or
flavonols in the molecule of which 40-OH is absent or substituted.