Connect a rotameter which has been previously calibrated to a primary standard to the vacuum supply (high volume pump). Disassemble the two halves of the BioSlS ™ and install a blank slide. Reassemble and connect a vacuum line from the rotameter to the male barbed fitting on the unit. (see figure 1). Turn the vacuum pump on and accurately adjust the vacuum level (flow) to suit your air characterization needs. Although a flow rate of per minute (lpm) is traditionally accepted, the BioSlS is capable of sampling rates of
5 to 50 lpm without damaging the slide. Consult with your local indoor air quality professional for appropriate sampling rates and times. Disassemble the BioSlS ™, remove the blank slide and pre-clean the top cap as directed within this guide.