The inoculum used for the stabilization of wastes was collected from the cow rumen of slaughtered cows in an abattoir in Abakaliki, Ebonyi State.
It was strained in cheesecloth and stored in an air-tight container, in order to keep the microorganisms in an anaerobic environment.
Anaerobic condition was maintained during the anaerobic digestion by sealing the mouth of the digesters.