Crude extracts were prepared from the powdered
leaves of each plant by sequential extraction
method using hexane, ethyl acetate and methanol
solvents. Leaf powder (1 Kg) of each plant was
soaked in 3L of Hexane for 48 h with
intermittent shaking. The extract was filtered
through Whatman No 1 filter paper, concentrated
in a rotary evaporator (Medica instruments
Mgf.Co. Sl.No:EV11.JF.012) and finally dried in
vacuum. The residue was soaked in other
solvents consecutively and extracted. All the
crude extracts were stored at 4oC in air tight glass
vials in the dark until used