2.3. Embrogenesis in ovule-derived calli
The stimulatory effects of carbohydrates on callus growth and embryogenesis were investigated in this experiment. Glycerol, lactose, sorbitol, mannitol, glucose and galactose were tested in the MT medium with various concentrations
(1–5%) of these saccarides. Cultures were incubated at 26 1 8C under light (43 mE m 2 s 1) with a 16 h photoperiod.