This research aims to study how to manage the waste, infections caused by small hospitals and laboratory experiment. Infectious waste management issues, there are also places restrictions on does not eliminate enough. Cause there are also illegal to throw waste transport operators from infections from the hospital and no hairs waste storage systems from a small hospital infections. From conceptual issues, so as to generate the disinfection with ozone, trash in high pressure plasma system disinfection in the infected waste from origin to be before removal. To reduce risk of infection to those exposed or those involved. From you the properties of ozone destruction and help inhibit the growth of germs. Cell protein encapsulated and moisten the germs, such as bacteria, viruses or fungi, the spores into the ozone will be destroyed. Make the germs cannot grow and the ozone is an unstable gas. After the ozone reaction will change as oxygen gas saphapklap which does not harm or affect human beings. Animals and the environment for this research in the first stage is to create a trash with ozone disinfection system in high pressure plasma test out ozone generators is 600 mg/hr and the machine can work continuously normal range 2 to 5 hours studying the relationship between the length of time that ozone exposure to infection.The bacteria E.coli ATCC 25922 and Salmonella Enteritidis can be sterilized using ozone as a percentage, is the same quantity that 600 mg/hr leads to find the correlation coefficient (R2) E.coli ATCC 25922 of bacteria and Salmonella Enteritidis infection 0.9588 equals equals 0.9864 shows that the relationship between a smash RA.The time that ozone exposure to E.coli bacteria to kill percentage in the ATCC 25922 and Salmonella Enteritidis. The relationship is very good. So, from the results of the trial, disinfection with ozone, trash in high pressure plasma system can help the E.coli ATCC 25922 to kill bacteria and Salmonella Enteritidis has 100 percent ozone dose 600 mg/hr used within 120 minutes.