Nested-PCR and TaqMan QPCR were performed in parallel
for detection and quantification of HHV-6 DNA. HHV-6 genomes
were amplified by nested PCR (nPCR) using the primer pairs
indicated in the Supplement material online. Virus isolates were
classified into HHV-6 A and HHV-6 B by direct sequencing. Further
details on PCR analyses are given in the Supplementary material
online.