Ascorbic acid was determined by the standard
method as described in AOAC (1990). The standard dye
solution for Ascorbic acid determination was prepared by
weighing 50 mg of 2, 6 dichlorophenol indophenol dye
and 42 mg of sodium bicarbonate, dissolving in hot
distilled water and volume made up to 250 ml. Ascorbic
acid standard solution was prepared by taking 50 mg of
ascorbic acid in 50 ml volumetric flask and the volume
was made up with 0.4% oxalic acid.