In this study, 38 isolates
were obtained with the MVYS culture medium. Between these,
strains LCC2-22, LCC2-27, LCC0-2, and LCC0-17 were
preselected for their high alcohol potential, with productivities
ranging between 2.4 and 2.5 g L−1 h−1 and yields above
0.45 g g−1, which are considered high enough for fermentations
in culture media with high organic acid content such as
vinasse. Of all possible sources of microorganisms in the distillery
environment, the molasses storage tank was the ideal
place to isolate suitable wild yeast strains due to an aggressive
environment that enables yeast to adapt for higher tolerance to
osmotic and acidic stress, which explains higher ethanol productivity
using molasses and vinasse as substrates