Transformants were selected on Luria Agar
media (LA)+Ampicilin (100 μg m-1) supplemented with X-gal
(40 μg ml-1). 16S-r-RNA gene in recombination plasmid library
(collection of 16S-rRNA genes in pGEM-T vector) were
amplified again using M13F and M13R primer (Moffett et al.
2000) to obtain individual 16S-rRNA genes.