ExoQuick can be considered an efficient method for exosomal RNA
analysis while being unsuitable for biochemical and immunodetection
analysis of protein content of the sample [9]. Possibility of amplification
of low abundant and specific target downstream to RNA isolation can
compensate for a scarce specificity of an exosome isolation step. However,
such unspecific precipitation method may prove to be confounding
if specific disease markers are sought in the serum, as these are
likely to be associated to specific vesicles sets, having either distinct
tissue/cell-type identity and/or subcellular origin, that cannot be separated
using neither method addressed in this study.