DISCUSSION
The specific combination of
auxin and cytokinin in culture media is
one of the most important factors for in
vitro regeneration of orchids. In this
study, we found that the most suitable
plant growth regulators for proliferation
and regeneration of V. lilacina were NAA
and BAP. Treatment with selective agents
is needed to avoid development of
undesirable numbers of escaped plants.
In this experiment, the selection agent
was hygromycin, an aminoglycoside
antibiotic that causes death to plant cells
by inhibiting transcription and translation
(Datta et al., 1999). We found that the
effective concentration of hygromycin