In the current study cellulase enzyme is isolated and its optimum conditions for production are determined. It is
found that maximum production of enzyme took place at the incubation period of 48hrs. The optimum temperature
of the enzyme was 45
o
C. The optimum pH at which the enzyme was stable was between 6.5&7.5. The optimum
substrate concentration for enzyme production was 1.5%.
Future work of the present work includes purification of enzyme in order to gain higher specific activity of cellulase
by the help of sophisticated purification procedures including Salt precipitation, Dialysis & Ion Exchange
chromatography.
The present study thus, reveals that agricultural waste harbours cellulolytic bacteria[9] that can be produced
commercially for its industrial applications and for the treatment of agricultural waste in the process of
bioremediation