Using the obtained results, we explain the ability of MPOCs
to penetrate only the first membrane layer, not the second
membrane layer, of the double layer liposomes (Figure 3b) as
follows: pore formation at the first membrane layer by MPOCs
resulted in lipid-adsorbed MPOCs inside the first layer of the
liposomes, and lipids on the MPOCs surface prevented the
particles to significantly adsorb more lipids from the second
membrane layer, thus inhibiting further pore induction.