This research aims to repair the character of drought tolerance at F1 hybrids of Jatropha curcas Linn. by in vitro selection
using polyethylene glycol. The seeds of J. curcas genotypes were sterilized before germinated on a sterile sand medium. The
zygotic embryos then grown in MS media + 0.5 mg·L–1 BAP + 0.4 mg·L–1 TDZ. The lethal dossage value was reached 73.57 %
from treatment of 15 % PEG, and 20 % of PEG treatment was reached 91.43 %. In selection media, the percentage of
death explants ranged from 76.00 % to 93.50 %. The callus that obtained, then propagated and developed into regeneration media
and expected could be developed completely plantlets which would have drought tolerance.