In addition, the sensitivity of the method is clearly linked to the
affinity of DRT primers used. Indeed, a primer with a poor affinity
for the targeted sequence, such as mix B, presents an obvious
difficulty to detect the transgene flanking regions in a given sample
submitted to food processing. Therefore, the importance of using
the four different DRT primers is highlighted in order to maximise
the detection power of the DNA walking method, independently
of the processing state of the tested matrix.