2.3. Characterization of silver nanoparticles
The synthesized colloidal silver nanoparticles were characterized
with the help of a UV–vis spectrophotometer (TU-1901). The
spectral background absorption was subtracted by using UV–vis
spectra of aqueous ALE.
X-ray powder diffraction (XRD) data was taken with a Ni filter
and Cu K radiation ( = 0.15406 nm), on the D/MAX-IIIC powder
diffractometer (Physics Co.), operated in the :2 mode primarily in
the 10–70◦ (2) range and step-scan of 2 = 0.02◦. The tube voltage
was 36 kV, and the tube current was 20 mA [11].
TEM measurements were performed on a JEOL TEM-2010
instrument (Japan) operated at an accelerating voltage of 200 kV.
On which there is a CCD photo recorder. A drop of silver colloid
was deposited on copper grid coated carbon membrane carefully
by micro-syringe for TEM and allowed the sample to dry at open
temperature in a self-designed room for 1 h [11].