W estern blotting (WB) is a powerful analytical tool that has been widely employed for decades in both scientific
research and medical diagnosis. In life science research, it is
often used to detect and determine target proteins in cells or
tissues.1−4 WB has also been applied to medical diagnostics,
such as blood testing for HIV5 or Lyme disease in blood
samples.6 WB has several advantages such as high sensitivity
and reliability7 but requires large amounts of target antigens
and specific antibodies for testing.